Coding

Part:BBa_K3763022

Designed by: Guokai Wang   Group: iGEM21_WHU-China   (2021-10-18)


coding sequence of mature PctA protein

BBa_K3763022 is the coding sequence for mature PctA protein (without Sec signal peptide). This sequence is used for the direct production of mature PctA protein, or to combinate with other parts (e.g. new signal peptide). Please note that this sequence do not have a initiation codon, so a signal peptide sequence or just a initiation codon should be added before the sequence is used for expression. The translation product of the gene is a 7kDa mature PctA protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 187


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Categories
Parameters
None